| Message: | Epigenetic inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases.Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. Inhibition of HMTs may lead to expression of the silenced genes and HMT inhibitors are currently developed for various therapeutic or experimental applications. ESET, G9a, SUV39-h1, SUV39-h2, SETDB1, Dim-5 and Eu-HMTase are histone methyltransferases that catalyze methylation of histone H3 at lysine 9 (H3-K9) in mammalian cells. H3-K9 methylation mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing gene expression at euchromatic sites. There is only the radioisotopic method currently available for measuring HMT activity/inhibition, which is time consuming, labor-intensive, and has low throughput or produces radioactive waste. The Histone Methyltransferase Activity/Inhibition Assay Kit (H3-K9) addresses these problems by using a unique procedure to measure HMT activity/inhibition.
https://www.creativebiomart.net/histone-methyltransferase-activityinhibition-assay-kit-h3-k9-463123.htm |
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