| Message: | Enteropeptidase (Enterokinase, EC 3.4.21.9) is a serine protease involved in activation of trypsinogen to trypsin, which in turn results in the activation of various digestive enzymes. It recognizes a highly specific amino acid sequence "DDDDK" and cleaves after the lysine residue. High specific activity of Enteropeptidase has been utilized in cleaving a variety of native or fusion proteins containing the above recognition. In PRSS7 Assay/Inhibitor Screening Kit (Fluorometric), we have utilized a peptide substrate containing the Enteropeptidase recognition sequence along with a fluorescent label "AFC". Enteropeptidase catalyzes the cleavage of this substrate and releases the AFC molecule, which can be easily quantified by measuring its fluorescence at Ex/Em = 380/500 nm. In the presence of potent Enteropeptidase inhibitor, the hydrolyzation of the substrate will be impeded. The kit provides a rapid, simple & reliable test for screening potential inhibitors of Enteropeptidase.
https://www.creativebiomart.net/prss7-assayinhibitor-screening-fluorometric-kit-463636.htm |
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