my account  log out
 

TR-FRET VDR Coactivator Assay Kit, goat

Create: 01/25/2019
Expired Date:never
Category: Diagnostic equipment [Health&Medical&Pharmaceutical]
Message:The TR-FRET Vitamin D Receptor (VDR) Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential VDR ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a VDR ligand-binding domain (VDR-LBD) that is tagged with glutathione-S-transferase (GST), in a homogenous mix-and-read assay format. The assay To run the TR-FRET VDR Coactivator Assay, VDR-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520: 495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound. Based upon the biology of the VDRcoactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide. https://www.creativebiomart.net/tr-fret-vdr-coactivator-assay-kit-goat-463148.htm
City:[US] 
Location:
 
Aug 25 2019Recombinant Human FGF2, None tagged429
Aug 25 2019Recombinant Human PVRL2, None tagged442
Aug 25 2019Recombinant Human PF4, None tagged453
Aug 25 2019Recombinant Human CXADR, Fc-His tagged470
Aug 25 2019Recombinant Mouse Cxadr, Fc-His tagged478
Aug 25 2019Recombinant Human CX3CL1, His tagged473
Aug 25 2019Recombinant Human CST5, His tagged429
Aug 25 2019Recombinant Human CPE, Fc tagged436
Aug 25 2019Recombinant Human Met, Fc-His tagged452
Aug 25 2019Recombinant Human CEACAM1, His tagged448

return back

Copyright © toextrade Inc. All rights reserved. Contact us