| Message: | Epigenetic activation or inactivation of genes play a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. G9a and polycomb group enzyme such as EZH2 are histone methyltransferases that catalyze methylation of histone H3 at lysine 27 (H3-K27) in mammalian cells. Tri-methylation of H3-K27 is a facultative heterochromatin mark which promotes the recruitment of polycomb group proteins for gene silencing. Increased H3-K27 tri-methylation is also found to be involved in some pathological processes such as cancer progress. There are only a couple of methods such as western blot used for measuring histone H3-K27 tri-methylation. The methods available so far are time consuming and labor intensive, or have low throughput. The H3-K27 Tri-Methylation Assay Kit addresses these problems by using a unique procedure to measure tri-methylation of histone H3-K27.
https://www.creativebiomart.net/histone-h3-k27-tri-methylation-assay-kit-463116.htm |
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