| Message: | Epigenetic activation or inactivation of genes plays a critical role in many important human diseases, especially in cancer. A major mechanism for epigenetic inactivation of the genes is methylation of CpG islands in genome DNA caused by DNA methyltransferases. Histone methyltransferases (HMTs) control or regulate DNA methylation through chromatin-dependent transcription repression or activation. HMTs transfer 1-3 methyl groups from S-adenosyl-L-methionine to the lysine and arginine residues of histone proteins. G9a and polycomb group enzyme such as EZH2 are histone methyltransferases that catalyze Methylation of histone H3 at lysine 27 (H3-K27) in mammalian cells. H3-K27 methylation mediates heterochromatin formation by forming a binding site for polycomb and also participates in silencing gene expression at euchromatic sites. Increased H3-K27 methylation is also found to be involved in some pathological processes such as cancer progress. There are only a couple of methods such as western blot used for measuring histone H3-K27 methylation. The methods available so far are time consuming and labor intensive, or have low throughput. The H3-K27 Methylation Assay Kit addresses theses problems by using a unique procedure to measure methylation of histone H3-K27.
https://www.creativebiomart.net/histone-h3-k27-methylation-assay-kit-463115.htm |
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