| Message: | Lysine histone methylation is one of the most robust epigenetic marks and is essential for the regulation of multiple cellular processes. The methylation of H3- K4 seems to be of particular significance, as it is associated with active regions of the genome. H3-K4 methylation was considered irreversible until the identification of a large number of histone demethylases indicated that demethylation events play an important role in histone modification dynamics. So far at least 2 classes of H3-K4 specific histone demethylase, LSD1 and JARIDs have been identified. LSD1 can remove di- and mono-methylation from H3-K4 by using an amine oxidase reaction, while JARIDs such as RBP2, PLU-1, SMCX, and SMCY catalyzes the removal of methylation by using a hydroxylation reaction and required iron and α-ketoglutarate as cofactors. H3-K4 specific demethylases are found to be involved in some pathological processes such as cancer progression. Inhibition of the enzymes may lead to re-methylation of H3-K4 and silencing of H3-K4 enriched active genes, there is few methods currently available for measuring activity/inhibition of H3-K4 specific methylases using a variety of cells/tissues. The Histone Demethylase (H3-K4 Specific) Activity/Inhibition Assay Kit uses a proprietary and unique procedure to measure activity/inhibition of H3-K4 specific histone demethylases using cell/ tissue extracts.
https://www.creativebiomart.net/histone-demethylase-h3-k4-specific-activityinhibition-assay-kit-463105.htm |
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