| Message: | Desheng Technology responded quickly and launched a highly efficient and stable virus nucleic acid storage solution in due course.
The components of the virus transporattion medium currently studied are mainly Hank's liquid foundation, gentamicin, fungal antibiotics, BSA (bovine serum albumin fifth component), biological buffer HEPES, amino acids, cryoprotectants, glycerin, etc. Minute
1. Hank's liquid:
Liquid A: Na2HPO4·2H2O 0.06 g KH2PO4 0.06 g MgSO4·7H2O 0.20 g Glucose 1.00 g NaCl 8.00 g triple distilled water 750 ml Liquid B: CaCl 20.14 g triple distilled water 100 ml
2. Gentamicin and fungal antibiotics: a combination of multiple antibiotics, has antibacterial and antifungal effects;
3. BSA (V): Bovine serum albumin (BSA), also known as the fifth component, as a protein stabilizer, is an albumin in bovine serum. It contains 583 amino acid residues and has a molecular weight of 66.430kDa. The point is 4.7. Bovine serum albumin has a wide range of applications in biochemical experiments. It can form a protective film on the protein shell of the virus, making it difficult to decompose, ensuring the integrity of the virus and increasing the separation rate; it can also be used in western blot As a Blocking agent; BSA is added to the digestion reaction buffer to protect the enzyme by increasing the protein concentration in the solution. Prevent enzyme decomposition and non-specific adsorption, can reduce the denaturation of some enzymes, and can reduce some unfavorable environmental factors such as heating, surface tension and chemical factors.
4. The neutral environment constructed by the biological buffer HEPES and HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) buffer helps increase the survival time of the virus and the stability of infection.
5. Amino acids: provide essential nutrients for virus survival. |
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