| Message: | Blood analysis is a very important and powerful diagnostic tool in animal health and welfare control. It is usually performed in higher vertebrates, and its reference value has been established, but fish hematology still needs further research. Many internal and environmental factors have a profound impact on the hematology value of fish, making it difficult to determine the reference value. In addition, due to the short clotting time, fish blood usually requires the addition of anticoagulants. The choice of anticoagulant is essential to obtain reliable blood test values. In this study, the effects of two common anticoagulants, K 2 EDTA (1.8 mg/ml) and lithium heparin (18 IU/ml), the hematological value of brown trout in the breeding season on the spawning season The spawner conducted an investigation.
The results of basic hematology analysis, such as compacted cell volume (PCV), hemoglobin concentration (HGB), red blood cell count (RBC), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and White blood cell count (WBC) was compared between K 2 EDTA and lithium heparin. Statistically significant differences were observed in PCV, MCV, and MCHC, while HGB, RBC, MCH, and WBC did not show such differences. These results indicate that lithium heparin provides more reliable results because the red blood cells in the K 2 EDTA-treated samples have a tendency to swell.
Lithium heparin is more suitable for hematological evaluation of spawning in trout than dipotassium ethylenediaminetetraacetate. Heparin does not affect the size of red blood cells, thereby minimizing the possibility of hemolysis. Compared with heparin, blood smears made from blood treated with EDTA are of higher quality. Based on current research, we suggest that K2EDTA can be used cautiously for blood count and HGB determination. In order to better understand the effects of anticoagulants on hematological parameters, further research is needed. |
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