| Message: | Luminol, is a yellow crystal or beige powder at room temperature and is a relatively stable chemical reagent. At the same time, luminol is a strong acid, which has a certain irritating effect on eyes, skin and respiratory tract.
It is one of the oldest and most commonly used reagents. It can be oxidized by peroxide under alkaline conditions and emit light at the same time. The redox reaction between luminol and peroxide requires a catalyst, which is generally more Valence metal ions, peroxidases such as iron, horseradish peroxidase, etc. This method is often used to detect the content of peroxides, heavy metals, peroxidase, and the derived free radicals, for toxicant analysis And analysis methods based on peroxidase and glucose oxidase.
Under normal circumstances, the chemiluminescence reaction between luminol and hydrogen peroxide is very rapid in the presence of certain catalysts.
Luminol Luminescence Principle
One is that sodium hypochlorite oxidizes luminol to make it glow;
The second is that hydrogen peroxide reacts with sodium hypochlorite to generate oxygen and oxidize luminol to make it glow:
The first is the equation for the reaction of sodium hypochlorite and hydrogen peroxide: NaClO+ H2O2== NaCl + O2+ H2O
Secondly, when luminol reacts with hydroxide, a double negative ion (Dianion) is formed, which can be oxidized by oxygen generated by the decomposition of hydrogen peroxide, and the product is an organic peroxide. |
my account
