| Message: | Tris buffer is a very important biological buffer, its advantages are as follows:
1. Tris base is relatively alkaline, so this buffer system can be used to prepare a wide range of pH buffers ranging from acidic to alkaline;
2. It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions;
3. High solubility in water and inert to many enzyme reactions;
4. High buffering capacity, generally used to stabilize the pH of the reaction system, with strong buffering capacity between pH 7.5-9.0;
5. Tris buffers are widely used and play an important role in biochemistry, molecular biology, in vitro diagnostics, cosmetics, coatings and other fields.
Matters needing attention when using Tris buffer:
1. The pH value of Tris buffer is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process;
2. Tris reacts with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals such as Cr3+, Fe3+, Ni2+, Co2+ and Cu2+, etc. It can work with heavy metals, but in some systems Also inhibits
3. It is easy to absorb CO2 in the air, and the prepared buffer should be tightly sealed;
4. It has certain interference effects on some pH electrodes, and electrodes that are compatible with Tris solution are required;
5. Tris is not suitable for bicinchoninic acid (BCA) determination;
6. Inhalation, ingestion or skin absorption can cause injury. Wear gloves and goggles during operation. |
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