| Message: | When performing biochemical reactions, a constant pH value is usually required. A good buffer can keep the pH constant and reach a stable working condition. In the process of cell culture, the growth and metabolism of cells will cause drastic pH changes in the environment of the culture solution. In order to maintain a stable cell growth environment, it is necessary to add an appropriate amount of biological buffer to the culture medium.
The following briefly introduces how the biological buffer maintains the pH value, because the buffer is composed of a weak acid (HA) and its conjugate base (A–) or a weak base and its conjugate acid. Weak acids and weak bases cannot be completely dissociated in water, but exist in a balanced state of dissociated and undissociated species in the buffer solution. For example, in an acetic acid solution, acetate ions, hydrogen ions, and undissociated acetic acid exist in equilibrium. Due to the dissociability of acetic acid (HAc), it can absorb H + or OH-. HAC can release H+ to neutralize OH- and form water. Conjugation base A-can react with H + ions added to the system to generate acetic acid.
There are countless biological buffers and alternative methods that can be used for cell culture in the market. Why choose HEPES, a biological buffer from Desheng? In fact, the suitable culture environment for most cells is pH7.2-pH7.4, and the application range of Desheng HEPES buffer just falls between pH6.8-pH8.2, which is in line with the pH value needed for cell culture. Compared with other buffers, HEPES has higher stability in maintaining the pH value of cell culture medium. Therefore, HEPES buffers are widely used in cell culture, tissue culture, protein purification and extraction, immunoprecipitation, Cell division, live cell imaging and other biological and biochemical research. |
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