| Message: | Tris is widely used in acute metabolic and respiratory acidemia, which is an alkaline buffer. It has a good buffer effect on metabolic acidosis and enzyme activity reaction, and may cause hypoglycemia, hypotension, nausea and vomiting. It can even inhibit breathing and stop breathing. Excessive intake or renal insufficiency can cause alkalescence. When Tris and acid titration reference material are used, the purity requirement will be very high, which is basically 99.9% - 100% Tris. Because Tris is weakly alkaline, when the temperature is below 25 ℃, PKA is 8.1, but according to the buffer theory, its effective buffer range is between ph7.0-9.2. The pH value of Tris aqueous solution is about 10.5, and hydrochloric acid is usually added to adjust the pH value to the desired value, but the effect of temperature on the pKa of Tris should also be noted. Because Tris buffer is a weak alkaline solution, DNA will be deprotonated in the solution, thus improving its solubility. Therefore, someone often adds EDTA to Tris hydrochloric acid buffer to make te buffer, and changes the solution adjusted by pH value to acetic acid to obtain Tae buffer.
|
my account
