| Message: | Advantages of Tris HCl buffer
1. The pH buffer in the range from acid to alkali can be prepared only by this buffer;
2. It has little interference to biochemical process and does not precipitate with calcium, magnesium and heavy metal ions.
Tris HCl buffer has the following disadvantages
1. The pH is greatly affected by the concentration. When diluted 10 times, the pH change is greater than 0.1
2. The temperature effect is large, such as pH = 8.4 at 4 ℃ and pH = 7.4 at 37 ℃
3. Easy to absorb carbon dioxide in the air
4. It can interfere with some pH electrodes, so it is necessary to use an electrode compatible with tris solution
In addition to Tris HCl, Tris has a variety of derivative buffers
1. TBS = Tris HCl + NaCl + KCl, which is often used to clean the immunostained tissue or Western blotting membrane;
2. Tbst = Tris HCl + NaCl + Tween20, which is commonly used as a membrane washing buffer in Western blotting;
3. TE = Tris HCl + EDTA, which can protect the base of DNA, is often used for DNA stabilization and storage;
4. Tae = Tris base + acetic acid + EDTA, is a widely used buffer system for short-time electrophoresis of large DNA fragments;
5. Tbe = trisbase + boric acid + EDTA, which is suitable for long-time DNA electrophoresis and has good separation effect for small fragments. |
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