| Message: | Generally speaking, nucleic acid extraction is a multi-step work. Firstly, it is necessary to break the biological sample materials such as cells and tissue materials, inactivate nuclease and release nucleic acid. The role of Tris, EDTA and other reagents is mainly reflected in the release of nucleic acid.
Nucleic acids are easy to hydrolyze in acid solution and stable in neutral or weak alkali solutions. Tris, namely trimethylaminomethane, has a pH buffer range of 7.0-9.0, which can maintain the stability of the nucleic acid released after the sample is cracked, so as to avoid the degradation of nucleic acid and improve the concentration and purity of nucleic acid.
EDTA, which can be combined with mg2+, ca2+, mn2+, fe2+, can prevent metal ions from activating protease, thus reducing the influence of metal ions on nucleic acid quality.
In conclusion, Tris, EDTA and other reagents can fully and effectively crack cells in the process of nucleic acid extraction, so that the nucleic acids in the cells can be released fully and get the nucleic acids with higher concentration, which is conducive to improving the quality of nucleic acids and ensuring the accuracy of subsequent operations. |
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