| Message: | According to the specific requirements of different experiments, the composition of the HEPESbuffer buffer and the preparation method are also different.
Usually HEPES is combined with sodium hydroxide NaOH or HEPES+ salt.
1. When preparing HEPES Stocksolution, first weigh HEPES and dissolve it in distilled water, and then use 0.5-1M To adjust the pH with NaOH, note that the effective pH buffer range is 6.8-8.2, then distilled water to a constant volume and store at 4°C.
2. When preparing HEPES buffer salt solution, you need to add sodium chloride, potassium chloride, disodium hydrogen phosphate, dextran, etc.Use NaOH to adjust the pH of the sample, and finally store it at a constant volume at a low temperature.
3. LeageneHEPES buffer, a commonly used solution in cell-cell adhesion experiments, is mainly composed of HEPES and chlorinated It is composed of sodium, potassium chloride, phosphate, glucose, etc., and contains Ca2+ and Mg2+ ions, pH 7.4, filtered and sterilized. Often It is used to clean tissues or cells (especially embryonic cells) in the process of long-term aggregation culture to protect the cadherin of the cells, and at the same time Does not affect the formation of cell aggregation. |
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