Message: | The first thing to introduce is the inactivated storage solution: the storage solution added with lysis salt is the inactivated storage solution, which usually contains Tris, lysis salt, EDTA, etc. The inactivated storage solution is safer to use, and the operating environment requirements are not so strict.
The main purpose of the inactivated preservation solution:
(1) The nucleic acid is lysed and released to facilitate subsequent real-time fluorescent PCR nucleic acid detection to determine whether the sample contains virus characteristic nucleic acid (that is, whether it is infected with the virus).
(2) The virus has a single structure and contains only one type of nucleic acid and protein. Therefore, when the characteristic nucleic acid is detected, the virus will be detected.
(3) The virus is a parasitic organism and cannot survive outside the body after sampling. If it cannot be detected in time, it needs to be placed in the virus preservation solution.
(4) In order to protect the safety of the virus detection environment, it is necessary to add cracking salt to inactivate the virus, and only need to release the nucleic acid that can be detected. |