| Message: | The molecular weight of Tris was 121.14:0.1mol/l, and the solution was 12.114g/l. Tris solution can absorb carbon dioxide from the air, so the bottle should be tightly closed. When Tris HCl solutions with different concentrations and pH values are prepared, a certain pH value of 0.05mol/l solution can be prepared first, and then diluted to the required concentration.
2、1M Tris-Hcl（PH7.4、PH7.6、PH8.0）
Component concentration 1m Tris HCl
Configuration quantity 1L
Configuration method:
1、 Weigh 121.1g Tris and put it in a 1 ﹣ l beaker;
2、 Add about 800 ml of deionized water and fully stir to dissolve;
3、 Add concentrated HCl according to the following value to adjust the required pH value;
PH value concentrated HCl
7.4 about 70 ml
6, about 60ml
0, about 42ml
4、 Volume the solution to 1 L;
5、 After high temperature and high pressure sterilization, store at room temperature.
3、1.5M Tris-Hcl（PH8.8）
Component concentration 1.5m Tris HCl
Configuration quantity 1L
Configuration method:
Weigh 181.7g Tris and put it in 1L beaker;
Add about 800 ml of deionized water and fully stir to dissolve;
Adjust pH value to 8.8 with concentrated HCl;
Volume the solution to 1 L;
After high temperature and high pressure sterilization, store at room temperature. |
my account
