| Message: | 1. Basic nature
The application of acridine compounds in chemiluminescence detection has the following advantages:
Low background luminescence and high signal-to-noise ratio;
Less interference factors in luminescence reaction;
Acridine compounds are used in chemiluminescence immunoassay (CLIA). That is, the antibody or antigen is labeled with acridine salt, and HNO3+H2O2 and NaOH are used as the luminescence priming reagent. Some add Triton X-100, CTAC, Tween-20 and other surfactants to the luminescence priming reagent to enhance luminescence.
Acridine ester DMAE-NHS or NSP-DMAE-NHS is an ideal chemiluminescence immunoassay labeling substance with good luminescence performance. Compared with the widely used acridinium ester AE-NHS, the luminescence intensity of the acridinium ester DMAE-NHS or NSP-DMAE-NHS and antibody (Ab) conjugates is higher, and the luminescence dynamics of the two are very similar. Both are flash-type luminescence and both reach the highest at 0.4s, but the thermal stability of DMAE-NHS or NSP-DMAE-NHS is significantly higher than that of AE-NHS. AE-NHS is only stable in acidic solutions, when pH>6.3 It is easy to hydrolyze later, but DMAE-NHS or NSP-DMAE-NHS is not. For example, at room temperature, it is very stable in a PB buffer with a pH of 7.0. After 16 days, the luminescence activity is only reduced by 3.6%. Their luminescence thermal stability with protein conjugates is similar to this.
The acridinium ester DMAE-NHS or NSP-DMAE-NHS is easy to label biomolecules and the luminescent activity and biological activity of the labeled compound are stable. The synthesized luminescent label is applied to chemiluminescence immunoassay with high sensitivity and other ideals. For example, the minimum detection limit of TSH is 0.007 mIu/L. Therefore, the acridinium ester DMAE-NHS or NSP-DMAE-NHS has good application value for the research of chemiluminescence analysis and chemiluminescence immunoassay.
3. Acridine Sulfonamide NSP-SA-NHS
Acridine sulfonamide is a very effective |
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